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Then, their cell constituents were homogenized by physical treatment using a tissue tearor in Trizol Reagent (Invitrogen, USA).
Each aliquot (0.6 g) and a gastric tissue sample (control) were disrupted using a Tissue Tearor (Biospec) in chilled RIPA-modified lysis buffer.
Extraction was performed by addition of Trizol reagent (Invitrogen, Carlsbad, CA) and subsequent homogenization using a Tissue Tearor (Biospec Products Inc., Bartlesville, OK).
The tissue was thawed for 15min then homogenized using a Tissue Tearor (Biospec Products, Bartleville, OK) for 15 s at 30,000 rpm.
Total RNA was isolated using mirVana® miRNA isolation kit (Ambion, Applied Biosystems CA USA) according to the manufacturer's recommendation Tissue homogenization was carried out using a tissue tearor (Model 985370, Dremel, WI, USA).
The density of aligned reads was calculated for mRNA (RefSeq exon [ 45, 46] as above. Mouse testis, brain, spleen and liver were harvested and flash-frozen in liquid nitrogen. Tissues were homogenized in TRIzol Reagent (Invitrogen) using a Tissue Tearor homogenizer (Biospec Products) and total RNA was purified according to the manufacturer's instructions.
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RNA was extracted from weight tissue samples by thawing in TRIzol Reagent (Invitrogen Corp. Carlsbad California USA) followed by immediate homogenization using a Tissue-Tearor homogenizer (BioSpec Products, Inc. Bartlesville Oklahoma, USA).
Colony counts were measured by dissecting tumors and organs from mice, homogenizing using a Tissue-Tearor (BioSpec), and plating serial dilutions on LB and LB Ampicillin plates.
After removal of the acetone, 0.6 ml TRI reagent (Ambion) was added to the antennae and the sample was homogenized using a Tissue-tearor (model 98370 365, Bartlesville, OK, USA).
Mouse spinal cord tissues were weighed and homogenized using a Tissue-Tearor homogenizer following the addition of 0.25% TFA solution at a ratio of 1 5 (w/v) [ 39].
The cord was placed in cold lysis buffer and homogenized on ice using a tissue homogenizer (Tissue Tearor 985370, Biospec Products, Bartlesville OK, USA).
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