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The tissue was weighed, rinsed with phosphate buffered saline and then homogenized using a tissue homogenizer.
After adding about 400 µl of cell lysate buffer, bone tissues were homogenized using a tissue homogenizer.
The lungs of mice were kept separately in the infecting medium (RPMI+5% FCS), minced mechanically using a mesh and homogenized using a tissue homogenizer to obtain a suspension.
In addition, the left lung or the whole lung, liver, kidneys, spleen and brain were all aseptically excised, washed in PBS and homogenized using a tissue homogenizer (Idea Works Laboratory Devices, Syracuse, NY).
Tissues were homogenized using a tissue homogenizer.
Organs were harvested and homogenized using a tissue homogenizer.
Brain tissue kept on ice was homogenized using a tissue homogenizer (OMNI international).
The powder was homogenized using a tissue homogenizer (Ultra-Turrax; Janke & Kunkel, IKA-Werke, Staufen, Germany) for 20 s in three volumes of ice-cold extraction buffer.
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Frozen tissue was homogenized using a Tissue Tearor homogenizer (Biospec Products) and small RNAs (10 – 200 bases) were purified using RNAzol RT (Molecular Research Center, Inc .. Purified RNAs were run on an 18 % denaturing PAGE, the region spanning ~22-35 ~22-35 excised, ant RNAs wasexcisedied for deep sequencing.
Tissues were homogenized in PBS using a Tissue Lyser homogenizer (Quiagen).
Tissue samples including the lesions (1.4 cm surrounding the inoculation site) were excised from the infected leaves and used for genomic DNA extraction using a Tissue Lyzer homogenizer and the DNeasy plant mini kit (Qiagen).
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