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We tested the prognostic value of ALDH1 using a tissue array of serous ovarian cancers, the most common histotype of human epithelial ovarian cancer.
Tissue blocks were constructed using a tissue array (Minicore; Alphelys Impasse Paul Langevin, Plaisir, France).
All TNBC cases (the 12 cases and the additional 36 cases using a tissue array) expressed MAS1.
We assessed expression of RUNX2 in a cohort of human breast cancers using a tissue array (TMA-1).
Tissue biopsies of 0.6 mm diameter were taken from each donor block and mounted in a recipient block using a tissue array machine.
A recent study [ 7] done using a tissue array consisting of 372 primary breast cancer specimens found a 5% incidence of FGFR2 amplification.
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The gastric TMA was constructed using a tissue arraying instrument (Beecher Instruments, Silver Spring, USA), as described previously [ 19].
The tissues (179 ovarian carcinoma, 40 borderline tumor, and 30 cystadenoma tissues) were sampled using a tissue arraying instrument (Beecher Instruments, Silver Spring, MD); a 0.6-mm-diameter cylinder of tissue was removed.
Using a tissue arraying instrument (Beecher Instruments, Sliver Spring, MD), each tissue core with a diameter of 1.5 mm was punched from the marked areas of intratumoral and peritumoral tissues and re-embedded.
Triplicate 0.6 mm diameter cylinders were punched from representative areas of an individual donor tissue block, and re-embedded into a recipient paraffin block in a defined position, using a tissue arraying instrument (Beecher Instruments, Silver Spring, MD, USA).
Duplicates of 0.6 mm diameter cylinders were punched from representative tumor areas of individual donor tissue blocks and reembedded into a recipient paraffin block at a defined position, using a tissue arraying instrument (Beecher Instruments, Silver Spring, MD, USA).
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