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Using a time cut-off, the Compliers at 18 hours and NonCompliers at 18 hours were then compared.
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Samples were assayed either immediately ("0" minutes) as a measure of rapid inactivation or after 60 minutes to simulate prolonged contact and allow comparability with previous studies using this time cut-off.
A sensitivity analysis was run for accelerometry-measured sedentary time, using a more conservative cut-off of <100 CPM.
Two hundred μl of each active fraction were concentrated 10 times using a 10 kDa cut-off concentrator, and loaded on a 10% SDS-PAGE in non-reducing conditions.
For total screen viewing time cut-off values, we used the American Academy of Pediatric guidelines of a maximum of 2 h per day [ 40].
The genes showing up-regulated expression and down-regulated expression following virus infection were compared at both times of infection using a 2-FC cut-off.
In a sensitivity analysis, we explored wider variations in the definition of time to adequate antibiotics (using time cut offs of 12 h, 24 h, 48 h and 72 h).
Volumes representing equivalent cell numbers were concentrated 10 times by Amicon centrifugation using a 10 kDa cut-off and 30 µl of concentrated medium was then separated on 10% SDS-PAGE containing 0.1% gelatin pre-casted gels (BioRAD).
Using a test cut-off time of 3 h instead of 5 h, 16% of fructose and 23% of lactose intolerance results would have changed from positive to negative.
Other studies investigating pre-hospital delay have used similar time cut offs [11].
We divided the diagnosis time into a binary outcome, i.e., delay and non-delay, by using a six-month cut-off point.
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