Our data study therefore adds to the limited data, using a similar tidal breathing maneuver to that used in the Danish study.
The other study compared a tidal breathing and breath-hold maneuvers (+/− velum closure) [ 18].
Cough reflex threshold to citric acid was evaluated with a tidal breathing nebulized solution delivered by an ultrasonic nebulizer (MU-32, Sharp Co. Ltd., Osaka, Japan) [ 19].
Cough reflex sensitivity to citric acid was evaluated with a tidal breathing nebulized solution delivered by an ultrasonic nebulizer (MU-32, Sharp Co. Ltd., Osaka, Japan) [ 10, 16].
Cough reflex sensitivity to citric acid was evaluated with a tidal breathing nebulized solution delivered by an ultrasonic nebulizer (MU-32, Sharp Co. Ltd., Osaka, Japan) [ 5].
Cough reflex challenge to citric acid was evaluated with a tidal breathing nebulized solution delivered by an ultrasonic nebulizer (NE-U17, Omron Co. Ltd., Kyoto, Japan) [ 17].
However, we consistently recorded lower readings using the tidal breathing maneuver.
Subjects with asthma and healthy control subjects in group 1 and 2 also underwent methacholine inhalation test using the tidal breathing method and allergen skin prick tests for Dermatophagoides pteronyssinus, dog, cat, and grass pollen.
Provocation tests were performed using a 2-minute tidal breathing method, adapted from Cockcroft and coworkers [ 18].
Methacholine inhalation challenge was performed as described by Cockcroft [7] using tidal breathing from a Wright nebulizer.
