We employed an intensity-based threshold algorithm that uses a threshold intensity P t to classify a pixel as particle or non-particle.
We determined alterations in gene expression in horny-head turbot liver relative to control fish by using a threshold of log2 intensity ratio ≥ 2. The MA plots revealed differential gene expression profiles between exposed and control turbot taken from sites in LACSD and OCSD, and an absence of significant differential gene expression in the control self self plots.
This routine detects particles using a threshold based on pixel intensity.
Using a threshold of 15% relative signal intensity enhancement after administration of intravenous gadolinium, Ho found in 74 patients with renal lesions a sensitivity of 100% and a specificity of 94% in the detection of renal cell carcinomas [ 17].
4) Using a thresholding, or image segmentation process, the intensity of pixels within the square is measured, and another rough nuclear region (binary image) is identified.
We use binning where each cell which contains one or more points is assigned value 1, while empty cells have value 0. Alternatively, we could use a threshold for point count, mean intensity, or a percentage of points within a vertical column [8, 42].
Bright pixels in the AIA 1700 Å images are flagged by using an intensity threshold of 3.5σ above the average intensity.
Scratch assays were quantified by generating still images from the time-lapse movie at specific time-points with Imovie (Apple Inc ., and scratch area measured by using a pixel intensity threshold with ImageJ software [42] that allowed exclusion of cells that had migrated into the scratch from the surface area calculation.
To assess p21 nuclear accumulation, p21 fluorescence was also measured within discrete nuclear regions as defined using a DAPI intensity threshold.
Masking biased probes using a signal intensity threshold of 80 increased the number of differentially-expressed genes identified in common bean by 2.8-fold (2,260 vs. 6,244).
