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As plasmid exchange is a time- and labor-consuming process, we attempted to improve the continuous crossbreeding method (Fig. 2b, c) using a third marker gene (AUR1-C; AUR-resistance).
Similar results were obtained using a third marker, the phosphorylation modification of H2AX.
Similar(58)
The levels of proliferation were further investigated using a second marker for cellular proliferation, Ki67, which had the benefit of less background staining.
We did not use a second marker for GVD; however, we were very careful to count only neurons containing two or more structures with a dense granule surrounded by the halo of a vacuole and it is unlikely that we have overestimated GVD.
To show that E2-Red/Green can be used as a third marker in conjunction with a green and a red FP, S. cerevisiae cells expressing DsRed-Express2, EGFP, E2-Red/Green, or no FP were grown separately and then pooled.
All samples were tested for HBsAg and anti-HCV markers by using a third-generation enzyme immunoassay (Axsym; HCV EIA 3.0; Abbott Laboratories, Abbott Park, Illinois, and HBsAg, Axsym) as previously published [ 14].
The protein content of the BALF and plasma were measured and the amount of BALF protein was used as a second marker of pulmonary permeability.
CD133 was used as a first marker for identification of colorectal CSC [ 4, 5].
Annexin A5 can visualize apoptotic PS externalization specifically, if used with a second marker showing an intact cell membrane [ 81].
[H]Paroxetine binding sites on the SERT of platelet membranes were used as a first marker of serotonergic function.
Mammaglobin expression was used as a second marker to quantify DEC in the BM of patients with breast cancer.
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