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Using the pLenti-CMV-DEST Gateway Vector (w118-1), we subcloned the wild-type (WT) or mutant coding sequences and generated lentiviruses using a Third Generation Packaging System in 293 T cells.
In this study, the wave climate in the East China Seas (including the Bohai Sea, the Yellow Sea and the East China Sea) for the past 35 years (1979 2013) is hind-casted using a third generation wave model – WAMC4 (Cycle 4 version of WAM model).
Briefly, HEK 293T cells were transiently transfected by calcium phosphate precipitation using a third generation lentiviral vector system [28], [29].
Lentiviral vectors were produced as described (32) in HEK-293T cells using a third generation packaging system.
CT scans of the chest, abdomen, and pelvis were obtained using a third generation General Electric scanner (GE Medical Systems, Milwaukee, WI, USA).
The HBsAg and the IgG antibodies to HCV markers were serologically assessed using a third generation enzyme immunoassay (Murex HBsAg Version 3, Abbott-Murex, South Africa) and (Murex, anti-HCV version 4.0 Abbott Kyalami, South Africa) respectively.
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The scans were performed using a fourth generation CT unit (Picker PQ 6000, Picker International, Cleveland, OH) under general anesthesia in sternal recumbency.
A new frequency compensation scheme using a second generation differential current conveyor (DCCII) for three-stage amplifiers is proposed.
The consistency of the VC mix was adjusted using a second generation (SNF) superplasticizer while self-consolidation characteristics of SCC were provided by a third generation (PCE) superplasticizer.
In our testing the app seemed to work well, even when using a first generation iPhone that doesn't have GPS.
Revision total knee arthroplasty using a second generation modular rotating hinge design was done on thirty two knees in 30 patients over an 8-year period.
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uses a third generation
using a Next generation
using a current generation
using a fourth generation
using a next generation
using a conformer generation
using a methodic generation
using a random generation
using a 4th generation
using a new generation
using a hydride generation
using a first generation
using a lead generation
using a second generation
using a small generation
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