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Chromatographic separation was performed on an HP-5MS fused silica capillary column (30 m × 0.25 mm × 0.25 μm), which was operated using a temperature programme as follows: the initial temperature was set at 60 °C for 2 min and was then increased by 10 °C/min up to 150 °C, 2 min isothermal, 3 °C/min up to 290 °C and held isothermal at 290 °C for 10 min.
FA methyl esters were eluted using a temperature programme set initially at 50°C for 2 min, increased at 20°C/min and held at 170°C for 1 min and then at 3°C/min and held at 212°C for 5 min to complete the run in 28 min.
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A helium carrier gas was used with a temperature programme set at 270°C – 290°C at a rate of 4°C min-1 with a 20 min hold.
Or, if using a temperature gauge, when it reads 50C/122F (for medium rare).
In order to obtain nanocomposite architecture membrane via pore plugging hydrothermal synthesis, the sealed autoclave was subjected to hydrothermal synthesis with interruption at 413 K using the temperature programme described in Daramola et al. (2015).
For primers using a touchdown programme the annealing temperature was decreased by 1 °C each cycle for the first 10 cycles, starting from 65 °C.
The products were analyzed by GC/MS using the same temperature programme as for the original extracts.
Core body temperature was assessed using a rectal temperature probe.
The stepper motor was controlled using a LabView programme to control the sample displacement.
Densitometry was performed using a documentation programme.
Randomisation will be performed using a computer programme.
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