Sentence examples for using a targeted pcr from inspiring English sources

Lazarus, K. L. & James, T. Y. Surveying the biodiversity of the Cryptomycota using a targeted PCR approach.

To investigate whether treatment with E2 had an impact on expression of genes known to play a role in angiogenesis, mRNAs expressed by uNK cells were assessed using a targeted PCR array (SAB biosciences).

AEI in this gene was confirmed using a targeted PCR-based SNaPshot method to measure allelic RNA ratios [ 3].

By using a targeted (PCR-based) metagenomic approach, we compared the presence and relative abundance of specific resistance determinants in the entire human gut microbiome before and after international travel.

This approach is closely followed by Strategy R, which also uses a targeted PCR screening with culture confirmation in the case of a positive PCR result, but carries out pre-emptive isolation precautions.

A second group was recruited using a targeted approach.

Use a targeted search.

This involved amplification of the targeted loci using a standard PCR protocol followed by digestion with the restriction enzymes PstI (NEB #R0140S) for rs12845494, and BsmAI (NEB #R0529L) for rs2506142.

RNA expression was then analyzed using targeted PCR arrays (SABiosciences) for Breast Cancer PAMM-1311), Cancer Pathway Finder (PAMM-033), Growth Factors (PAMM-041), and Inflammatory Cytokines and Receptors(PAMM-011).

Primers flanking the targeted exons (sequence on request) were used to amplify the cDNA as described previously for dystrophin, but using a single PCR for 35 cycles.

Real-time PCR was performed using a TaqMan PCR kit and an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems).