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For fixation, metacestode vesicles were gently opened using a syringe tip to allow entry of the fixative and detection reagents.
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The guidewire was then withdrawn, and pancreatic juice was collected using a syringe with the tip of the cannula in the MPD.
Care was taken to apply the phosphoric etching gel precisely on the implant surface using a syringe with a small tip.
At the end of each immersion period, 5 ml of eluent was removed from each solution using a syringe with a plastic tip.
Polymer solutions were fed into the needle tip using a syringe at a flow rate of 0.9 ml/h; in a drum collector (50 mm wide and 70 mm thick) covered with aluminum sheet.
The PCL solution was loaded into a 10 ml syringe and extruded through an 18-gauge blunt tip needle at 3.0 mL/h using a syringe pump (PY2 70-2209; Harvard Apparatus, Holliston, MA).
Briefly, PCL solutions (in chloroform and 5 10 wt % concentrations) were sprayed at 0.2 or 0.5 mL/h using a syringe pump at 10 18 kV and collected on aluminium foil at varying tip-to-collector distance (15 25 cm).
For preparation of fibers, outlet flow rate was maintained at 0.4 mL h−1 using a syringe pump, and electrospun fibers were collected on an aluminum foil kept at a gap of 15 cm from capillary tip.
Samples were manually introduced to the instrument using a syringe.
Such variations were unlikely to be caused by the experimental procedures because the tip of the needle had been carefully placed near the centre of all tumours and the infusion protocol had been standardised by using a syringe pump.
A syringe tip connected to an injection pump was used to flow deionized water into the chamber with a rate of 20 ml/h.
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