Sentence examples for using a synthetic substrate from inspiring English sources

Trypsin activity was verified using a synthetic substrate.

Using a synthetic substrate based on the human fibrinogen A-alpha polypeptide sequence as an indicator of enzymatic activity, salmon and human thrombin preparations contain similar specific activities per mass of purified protein.

The enzyme activity was checked using a synthetic substrate [ 4].

Hexosaminidase activity in the culture supernatant was determined using a synthetic substrate as described below.

Aminopeptidase assay was carried out using a synthetic substrate l-alanine-4-nitroanilide [ 48] with slight modifications.

The neutralizing antibody assay is based on a colorimetric enzyme activity assay using a synthetic substrate 4-nitrophenyl-β-D-glucopyranoside and quantifies the ability of anti-imiglucerase or anti velaglucerase alfa antibodies to inhibit their enzymatic activities in vitro.

The enzyme activity was determined at 50 °C using a synthetic chromogenic substrate, 4-nitrophenyl N,N′-diacetyl-β-d-chitobioside [4NP-(GlcNat)2], at a concentration of 400 μM, as previously described (Honda et al. 2016).

We also tested the effect of autophosphorylation on enzymatic activity, using a synthetic peptide substrate.

We tested for tyrosine kinase activity using a synthetic Src substrate peptide.

Acetylthiocholine iodide was used as a synthetic substrate that was hydrolyzed to produce thiocholine that in turn reacted with a 5,5'-dithiobis 2-nitrobenzoic acid) to yield a product measurable spectrophotometrically at 412 nm.

We also tested CtpAp cleavage on this substrate using a synthetic oligopeptide (S24) comprising 24 aa (Val330 to Gly353) of the pD1 C-terminus.