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Xylitol produced by a strain of Candida guilliermondii was obtained using a synthetic medium containing D-xylose in a continuous membrane bioreactor.
The biofilm cultures were investigated under controlled turbulent flowing conditions and nutrients were provided using a synthetic medium (tap water supplemented with nitrate, phosphate and silica) to favour the biofilm growth.
A small-scale (1.5 L) bioethanol fermentation was conducted using a synthetic medium comprising yeast, water, sugar (150 g/L glucose and 30 g/L xylose), yeast extract (10 g/L) and peptone (20 g/L).
Although the present bioethanol fermentation was performed using a synthetic medium, the impact of using an actual lignocellulosic hydrolysate or other complex fermentation medium on the quantification of ethanol in the headspace would be anticipated to be minimal.
Using a synthetic medium and phosphate limitation, C. acetobutylicum could be kept in a steady state of acidogenesis when the pH was >5 and in a steady state of solventogenesis at pH 4.3 for at least several months (Bahl et al. 1982a, b).
One approach for gaining a deeper understanding of the interactions between inhibitory components present in biomass hydrolysates and microorganisms, including inhibition synergies, levels of inhibition, and metabolic effects, involves using a synthetic medium that mimics the composition of authentic lignocellulosic hydrolysates, that is, a synthetic hydrolysate (SH).
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We performed column experiments using a synthetic porous medium, in which a substantial part of the sorption sites was concentrated in regions within small grained aggregates that were accessible only by diffusion, a feature often found in natural soils and sediments.
A 2 × 3 factorial design was employed using a synthetic grape juice medium with YAN (150 or 250 mg N/L) and equal proportions of glucose/fructose (230, 250, or 270 g/L) as variables.
Time course of (a) substrate utilization, (b) growth and formation of (c) ethanol and (d) arabitol and e) DO level during batch cultivation of D. nepalensis NCYC 3413 in 2.5 L bioreactor using a semi synthetic medium containing 150 g/L glucose, agitated at 400 & 700 rpm and aeration level was maintained at 0.5 vvm.
The Propionibacterium acidipropionici ATCC 4875 used in this study was grown in a synthetic medium using 10 g L−1 sorbitol as a carbon source, 10 g L−1 yeast extract, 1 g L−1 KH2PO4, 2 g L−1 (NH4)2HPO4, and the following micronutrients: 5 mg L−1 FeSO4.7H2O, 10 mg L−1 MgSO4.7H2.5 2.5 mg L−1 MnSO4.H2O, 5 mg L−1 ZnSO4.7H2O, 10 mg L−1 CaCl2.2H2O, 10 mg L−1 Coral.6H2O (Coral et al. 2008).
The highest values for ethanol yield (approximately 60% of theoretical maximum ethanol) were achieved in assays A3, A5 and A15, which were similar to that obtained using a semi-synthetic medium.
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