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Plasmids were sequenced by Beckman Coulter Genomics using a synthesised primer complimentary to an internal site in eGFP (GGCCGTTTACGTCGCCGTCC) and standard primers complementary to the T7 promoter and M13 reverse.
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Plasmid DNA (pUC19 and pBR322) was sequence-specifically, covalently labelled with Cy3 fluorophores using a newly synthesised N-adenosylaziridine cofactor and the DNA methyltransferase M.TaqI.
Smith, D; Leach, M; Kellner, A, Indirect holographic imaging of antennas using an electronically synthesised "slow-wave", Ieee Antennas and Propagation Society, Ap S International Symposium (Digest), vol. 1 (September, 2004), pp. 703-706 [abs].
We devised a simple approach of growing vectors using a flow field synthesising approach.
Ideally, it could be studied in a clinical trial or, alternatively, using a model which synthesises the evidence from all available sources and levels of evidentiary quality [ 19, 20].
Cu2O nanocubes were synthesised using a previously reported seed-mediated approach1,3.
Bundles of single-wall carbon nanotubes (SWCNT) were synthesised using a chemical vapour deposition technique.
A molecularly imprinted polymer (MIP) selective for tylosin was designed and synthesised using a computational method (MIP "dialling").
Initially the mixed oxide nanopowder, containing 25 wt% gallia, was synthesised using a facile cost effective sol-gel procedure.
Total RNA was extracted from cells using Isogen II (Nippongene) and cDNA was synthesised using a Transcriptor First Strand cDNA Synthesis Kit (Roche) according to the manufacturer's instructions.
It is demonstrated that a reflection spectrum with desired number of channels can be easily synthesised using a general multi-variable optimization method.
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