Sentence examples for using a sucrose gradient from inspiring English sources

The SEC weight molecular size distributions indicate that the extraction procedure with protease is most effective in obtaining pure phytoglycogen from grain, whereas that without protease at cold temperature followed by purification using a sucrose gradient is more effective for leaf material.

Furthermore, the ease of separation of AuNPs using a sucrose gradient centrifugation is an improvement to research using biological methods for nanoparticle fabrication.

Exosomes were purified using a sucrose gradient where, the 100,000g pellet was re-suspended in 0.5 ml of 0.25 M sucrose (20 mM HEPES/NaOH, pH 7.2) and layered on top of a linear sucrose gradient (0.25 2.0 M sucrose, 20 mM Hepes/NaOH, pH 7.2).

Mitochondria and MAM were purified using a sucrose gradient.

Cysts were purified from feces using a sucrose gradient centrifugation as in [ 42].

Native chromatin was isolated using a sucrose gradient from HEK 293T cells.

In order to find out, we used a sucrose gradient protocol, followed by hypotonic lysis of synaptosomes to prepare synaptic membranes and synaptic vesicles [20].

Their protocol is similar to our Protocol C, in that they use PVP and β-mercaptoethanol for prevention of polyphenol oxidation, it differs because they use a sucrose gradient for nuclei isolation and silica column for DNA isolation.

To verify that the identified miRs are associated directly with exosomes, we separated the mutp53-derived exosomal isolations into fractions using a sucrose density gradient technique.

All three protocols first separated synaptic membrane fractions (SPM) using a sucrose density gradient.

Three days later, cells were harvested, broken up in a Douncer, and fractionated using a sucrose step gradient, as described [20].