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The results demonstrate predictive skill using a stringent validation procedure, however, indicating strong model performance even with this relatively limited observation dataset.
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To minimize false discovery, single-peptide hits among the 1520 provisional proteins were filtered using a stringent multistep cross-validation process as outlined in Methods S1.
On the other hand, HMM_RA is evaluated using a stringent ten-fold cross-validation.
PYCARD therefore represented a stringent validation test of the methylation arrays.
We used a stringent SVM score threshold of 0.5 to reduce the number of false positives.
We use a stringent threshold (score > 0.9999) to assign a sample as a VINO.
The workflow is greatly simplified for clarity and therefore the model should not be used without stringent validation.
Recently, a more stringent distance of 500 bp was used, and we will also use this more stringent validation for reference to earlier work (Abeel et al., 2008; Goni et al., 2007).
Like many other pharmacovigilance data analyses, our proposed associations between ADEs and high-dimensional drug interactions need further molecular experimental validation, and using a more stringent pharmacoepidemiological study design and alternative databases.
Using a cross validation scheme yielded AUROCs of 0.82 for the derivation and 0.79 for the validation sets.
The high stringent condition was chosen to achieve a more complete isolation of individual paralogues, orthologues, and homoeologues compared with using a low stringent condition.
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