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A relationship between SNP in the 1 kb upstream region and differential expression was only observable when using a stringent test of differential expression (absolute log2 fold change > 0.5; pplr < 0.005).
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The number of mice in this experiment was, however, too small to achieve statistical significance using a stringent log-rank test (p = 0.1052).
Percentage variance accounted for (%V), together with analyses of residuals, is used as a stringent test of accuracy of prediction against observed data.
Stable transfection of a 150-kb BAC carrying oriP/EBNA-1 was used as a stringent test of the ability to transfect such large DNA intact into mammalian cells in tissue culture.
Since 803 unique patterns were tested for associations, we corrected for multiple testing using a stringent p-value of 10−5 (equivalent to a p value <0.01 after Bonferroni correction).
In fact, after controlling for multiple testing and using a stringent significance criterion (FDR < 0.01), we were able to identify a set of 3,078 trustworthy miRNA-target relations involving 117 (39%) of 309 selected miRNAs.
Thus, we sought to validate the identified network and approach using a most stringent test: interrogating the network to discover a treatment that produced a capability not reached in any of the previous experiments in this field.
However using an additional stringent test of selection (M8a) all 23 CPGs including the procyclin CPG showed significant evidence of positive selection at p<0.05 following Benjamini-Hochberg correction, thus we consider these 23 CPGs to have significant evidence of positive selection.
It may also be used to put to a stringent test different experimental criterion to measure the nonclassical character of a state.
Here we used a stringent branch-site test to detect positive selection (see Methods).
Attempts to design functional hybrid PKSs using this loading module provide a stringent test of our understanding of PKS structure and function, since the order of the domains in the loading and first extension module is non-canonical in the soraphen PKS, and the producing strain is not an actinomycete.
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CEO of Professional Science Editing for Scientists @ prosciediting.com