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Using a stringent staining paradigm, serially adjacent 7 µm sections containing substantia nigra were processed with H&E (Figure 2A), an antibody against total Elk-1 (Figures 2B), and an antibody against alpha-synuclein (Figure 2C).
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Therefore, we deemed using a stringent protocol for c-Myc immunohistochemistry indispensible for reliable c-Myc staining.
The identification of CNV regions (CNVRs) was performed by using a stringent procedure.
We report these results using a stringent family-wise error (FWE) volume-corrected threshold of P<0.05.
ClCE8 homologs were retrieved from the NCBInr protein database using a stringent E value of 1e-50.
The hit rate using a stringent cutoff of 2-fold inhibition (activity of <0.5) was 0.05%.
Using a stringent filtering method, we identified 3.1 million SNPs, 193 thousand INDELs, and 282 CNVs.
The gene expression differences of the categories were determined using a stringent default value.
On the other hand, HMM_RA is evaluated using a stringent ten-fold cross-validation.
Using a stringent cut-off, we identified 3,478 differentially expressed genes (DEGs) in response to EMT.
DOI: http://dx.doi.org/10.7554/eLife.01828.004 To focus our analysis, we used a stringent cut-off of a ninefold increase in HPL staining intensity, which resulted in 19 genes.
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