Sentence examples for using a stringent nucleotide from inspiring English sources

Next, to reduce the identification of false positive SNPs, we filtered potential SNPs using a stringent nucleotide depth cutoff of 10 [e.g., at least 10 adenines (A) in one genotype vs. at least 10 guanines (G) in the other genotype] for each genotype (see Methods for details).

Using a stringent filter, we identified ∼7.6 million single-nucleotide polymorphisms (SNPs) and 8,839 copy number variations (CNVs) in the mapped regions; 42% of the SNPs have not found in other chickens before.

Using a stringent criterion of 0 mismatches within the 16 nucleotide tag alignments, most of the tags aligned to the models but large numbers of tags did not.

Using a stringent pipeline, we identified an average of 127,347 single nucleotide polymorphisms (SNPs) per strain using the SAMtools software [ 11], ranging from 40,925 to 392,544 (Table  1; Additional file 2: Figure S2).

Using a stringent cutoff requiring sequence identity of at least 90% and a minimum alignment length of 30 nucleotides, 509 (38.6%) sequences in our library have a hit in the previous library, and 711 (54%) of the sequences in the earlier library have a hit in ours.

The identification of CNV regions (CNVRs) was performed by using a stringent procedure.

We report these results using a stringent family-wise error (FWE) volume-corrected threshold of P<0.05.

ClCE8 homologs were retrieved from the NCBInr protein database using a stringent E value of 1e-50.

The hit rate using a stringent cutoff of 2-fold inhibition (activity of <0.5) was 0.05%.

Using a stringent filtering method, we identified 3.1 million SNPs, 193 thousand INDELs, and 282 CNVs.

The gene expression differences of the categories were determined using a stringent default value.