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Tau aggregation was further confirmed by immunocytochemical (ICC) analysis using a stringent extraction protocol with TX-100, extracting soluble forms of Tau from the cells, while only aggregated forms of Tau remained detectable.
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The identification of CNV regions (CNVRs) was performed by using a stringent procedure.
We report these results using a stringent family-wise error (FWE) volume-corrected threshold of P<0.05.
ClCE8 homologs were retrieved from the NCBInr protein database using a stringent E value of 1e-50.
The hit rate using a stringent cutoff of 2-fold inhibition (activity of <0.5) was 0.05%.
Using a stringent filtering method, we identified 3.1 million SNPs, 193 thousand INDELs, and 282 CNVs.
The gene expression differences of the categories were determined using a stringent default value.
On the other hand, HMM_RA is evaluated using a stringent ten-fold cross-validation.
Using a stringent cut-off, we identified 3,478 differentially expressed genes (DEGs) in response to EMT.
We used a stringent SVM score threshold of 0.5 to reduce the number of false positives.
We use a stringent threshold (score > 0.9999) to assign a sample as a VINO.
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