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We systematically characterize sources of variation in the application of direct MS to the analysis of whole serum and address computational issues using a stringent approach to the analytic methods developed for this study, guided by a series of simulation experiments (Billings, E, unpublished data, 2006).
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We used a stringent approach and extracted only the regions of kiwi coding sequences that had at least 90% identity to those of chicken.
We used a stringent approach in methylation profiling by filtering out CpG probes that overlapped repeats or known SNPs, which might have produced unreliable results.
Using a stringent statistical approach, we identified 191 genes the expression of which was significantly and consistently different in both ρ° cell lines compared with their parental cell lines.
A total of 892,803 SNP polymorphisms were identified among the ten accessions of B. napus, using a stringent filtering approach favouring high quality SNPs over exhaustive SNP sampling to provide a resource of immediate value for crop improvement.
Thus, it was felt that valuable information was lost using such a stringent approach.
Using such a stringent approach, we identify an initial set of transcripts whose expression levels show low intra-individual variation and large inter-individual variation and are sufficiently heritable to make them candidate expression phenotypes for eQTL mapping.
Thus, we conclude that for these wheat data, a robust physical map can be obtained by WGP when using a stringent stepwise, cut-off approach starting at 1e-75 and ending with a final cut-off of 1e-11.
First, concordant results were obtained when using different analytical approaches and when using a stringent clinical phenotypic endpoint namely AIDS (1987 CDC criteria).
Using a more stringent approach of orthology revealed that for the largest gene indices of potato and tomato, a putative ortholog could be identified at the nucleotide level for 47% of the unique transcripts in the gene index.
Using a more stringent approach in terms of consistent GO reconstruction and phylogenetic certainty (node 6, Aculeata an undisputed clade), the ant1GO occurred at least 160 150 Ma in the late Jurassic period.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com