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Only half were reproduced successfully using a strict threshold for significance that was set at P < 0.0001 (the P value is a common test for judging the strength of scientific evidence).
Using a strict threshold of q < 10-10, we identified 22,554 overlapping binding sites.
Using a strict threshold of significance, there are 51 EST showing a difference in expression by behavioral type (d.f. 1,>36; log P < −5); 23 of these show a greater than twofold difference (Fig. 3).
DOI: http://dx.doi.org/10.7554/eLife.08274.004 10.7554/eLiFigure74.005 Figure 1 figure supplement 2. c-Fos+ cell density in the IL calculated in the analysis using a normal threshold and in an additional analysis using a strict threshold.
Using a strict threshold of 95% CI resulted in acceptable false positive rates for all statistics, whereas a liberal threshold of 90% CI resulted in reduced specificity (in particular for the base-to-peak and peak-to-peak P300 statistics), without much increase in sensitivity.
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Because of the jitter or variability in localization errors, using a stricter threshold might lead to erroneous exclusion of some trials in which participants reported the location of another object but in a relatively imprecise manner.
Therefore, we chose to use a strict threshold to detect and subsequently analyze only high-confidence p53 peaks.
Given the large number of tests for association performed and the subtle effects of naturally occurring variants on olfactory behavior, none of the polymorphisms were significant using a strict Bonferroni-corrected significance threshold.
Individual SNPs were tested for both allelic and genotypic associations by calculating Fisher's exact p-values and using a strict Bonferroni-corrected 0.05 genome-wide significance threshold of 7.1 × 10-8 (α = 0.05/705,969).
The 188 genes used in the survival signature were selected by using a stricter P-value threshold, 0.01.
We used a strict Bonferroni threshold (based on the number of SNPs and the number of miRNAs tested, p < 10-10) to define a significant "trans" association.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com