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This analysis revealed that irradiation alters the expression of 17 miRNA species using a strict cutoff of p<0.01 for significance.
Similarly, find anti-correlated pairs of down-regulated microRNA and up-regulated mRNAs in normal kidney samples using a strict cutoff.
Step 3: Using TargetScan, retain only the mRNA in Step 2 which have a conserved seed sequence in their 3' UTR for at least one of the microRNA from Step 1. Step 4: Find anti-correlated pairs of up-regulated microRNA and down-regulated mRNA in ccRCC samples using a strict cutoff (P0) in Pearson correlation coefficient in ccRCC samples.
Because even small genomic variations may occasionally change the functions of genes, we filtered the original 5097 LCBs using a strict cutoff value (1.01), which means the length of gaps in each LCB is smaller than 1% of the length of the non-gap regions.
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Also, using a stricter cutoff for poor SBSOD left us with too few of those participants willing to participate in the experiment.
Note that similar results were observed for a subset of proteins that did not share homology using a stricter cutoff (HSSP-value<0, Fig. S1).
To avoid misinterpretation, we used a strict cutoff and excluded all the structures with a P > 0.95 and a z score < −4.
Based on a sequence homology searches within each OG against the NR database using a strict E-value cutoff of 10−120, 5,018 genes were annotated across the GO categories.
Using a strict 10-30 e-value cutoff and keeping only one hit with the longest alignment within a blasted BES, we found 9835 unique BES hits for 4152 unigenes with ≥95% sequence identity over regions ≥100 bp in length of the aligned sequences.
We use a stricter cut-off for research purposes (8.0 mmol∙l−1).
Others have used a strict PSA cutoff.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com