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Using a strain harboring identically myc-tagged Est1 and Est2, we found that Est2, in addition to Est1, co-immunoprecipitated with flag-tagged Yku80.
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For example, Buchan and colleagues recently used a yeast strain harboring Pab1-GFP and Edc3-mCherry as SG and P-body markers.
A strain harboring pSP-GM1 (AB10) served as negative control.
Strong mitotic Y2H interaction was generated by using the same strain harboring plasmids coding for the Mei4 and Rec114 fusion proteins, whereas a strong meiosis-specific Y2H interaction was generated in a diploid harboring plasmids coding for the Spo11 and Rec104 fusion proteins (Arora et al. 2004).
To obtain a wide range of total cellular FtsZ levels, we used the B/r A strain which harbors the ftsZ-mEos2 fusion gene under the control of the lac promoter on a multicopy plasmid pCA24N and employed a "serial dilution by division" strategy.
To this end, a number of phenotypic assays were completed using a series of strains harboring wild-type H2B, H2B K37A, H2B K37R, or H2B K123R mutant histones (in most cases, except where specifically noted, the H2B K123R mutant strain was included as a positive control).
E. coli ATCC 352183 was used as positive strain harboring blaTEM gene [ 9].
By using only strains harboring the locus, we ignore the loss or gain of the whole CRISPR/Cas system.
The gene was identified using a congenic strain with E3 alleles between 23.476-23.730 Mb and harbors three genes.
The feasibility of using the mutant strains harboring a selection of expression plasmids for the production of PAC was evaluated.
Therefore, we repeated our analysis of sspA-S47 mutant alleles using strains harboring a deletion for sspB.
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