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A volume 30 ml of DI water was stirred rapidly in a conical flask using a stirrer bar.
Then, 75 ml of the enzyme BAN (Bacillus amyloliquefaciens α-amylase) from the Novozymes company (Bagsværd, Denmark) and 75 g of CaCl2 were added and stirred for 20 min, using a stirrer velocity of 137 rpm with a pitched 3-blade impeller.
Dissolved oxygen was kept constant at around 80%%, by using a stirrer (100 rpm) and injection of air (0.1 bar).
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Fermentation conditions used a stirrer speed of 800 rpm, with pH of 5.0 (maintained by automatic addition of 2 N potassium hydroxide) and a dilution rate of 0.05 hr−1.
Use a stirrer and stir everything well.
Agitation was continued at 270 rpm using a magnetic stirrer (stirrer bar: length 40 mm, ∅ 0.8 mm) of Tarson, India (model: Spinnot).
When the temperature of the emulsion reached 40°C, the organoleptic ingredients such as color and fragrance were added in to the emulsion with general mixing using a mechanical stirrer (Ika, Eurostar digital overhead stirrer).
In brief, 50 mg of polymer was dissolved in 2.5 mL ethyl acetate for 2 h at ambient temperature using a magnetic stirrer (Cimarec i Poly 15 Multipoint stirrer, Thermo Electron Corporation, Beenham, Reading, UK).
At a nearby table, Cornelia had cut a tiny chunk out of each of four lipsticks, using a wooden stirrer to smear them onto little white squares of paper.
In contrast, substantial transfer of PBDEs to dust was detected when the TV casing was abraded using a magnetic stirrer bar.
The solution was thoroughly stirred using a mechanical stirrer.
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