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Each well was inoculated with a single His+ transformant using a sterile toothpick and stirred to resuspend the cells.
Briefly, cells from a pure colony were placed on the clean area of the slide using a sterile toothpick and a drop of the PROLD reagent was added.
Bacteria were transferred from the duplicate patch plate directly into the PCR tube using a sterile toothpick.
Before infiltration, a small incision was made at the site of infiltration using a sterile toothpick to enhance the efficiency of infiltration.
After treatments with M. alternifolia, biofilm cells were scraped off the well wall using a sterile toothpick and transferred to Falcon tubes containing 10 mL PBS.
Bacterial cells from an isolated colony was point inoculated into the centre of a swim plate or on the surface of a swarm plate using a sterile toothpick.
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Cells from an exponential phase aerobic culture were collected by centrifugation and the resulting cell pellets were sampled using a sterile square toothpick, to inoculate the plate-agar interstice.
Plates were inoculated by picking a colony using a sharp sterile toothpick and stabbing to the bottom of the plates, which were incubated at 37°C for two days.
For S. cerevisiae, patches of strains to be tested were incubated on YPD plates or SPO plates and then a sterile toothpick was used to make a patch of the yeast on the agar surface of a 50 mm petri dish containing 10 ml of 2% agar.
For each sample 30 positive colonies were picked with a sterile toothpick, diluted in 100 μl Sabax water (Adcock Ingram) and used directly as DNA template for PCR.
The medium was inoculated with a small amount of NTG powder by a sterile toothpick.
More suggestions(15)
using a sterile probe
using a sterile water
using a fresh toothpick
using a sterile stir
using a sterile needle
using a sterile catheter
using a sterile stirrer
using a sterile template
using a sterile pipette
using a sterile pencil
using a sterile spreader
using a sterile cork
using a sterile tissue
using a sterile ruler
using a sterile magnet
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