Thereafter, the larvae belonging to the different groups were separately placed into glass test tubes (10 ml) including 3 ml nutrient broth (Difco, NJ, USA) with sterile forceps and completely homogenized, using a sterile glass tissue grinder.
Resected breast tissue was transferred immediately to the pathology department upon completion of mastectomy, where pathologists resected slices of breast tissue using a sterile technique.
Lesions were carefully removed without contamination from surrounding tissue using a sterile technique with a 27-gauge needles (Davies Health Care) and each lesion placed separately into separate sterile Eppendorf microcentrifuge tubes.
Eight 10 μm curl sections were cut from formalin-fixed paraffin-processed blocks of lung including at least 75% tumor tissue, placed in a 1.5 ml microcentrifuge tube and heated at 70°C in a Stuart SBH200D heating block for 20 min to allow excess paraffin wax to be removed from the tissue using a sterile fine tip plastic pasteur pipette.
Colonies of hESC in feeder-free conditions were removed from the tissue culture plates using a sterile cell scraper and partially dissociated by gentle pipetting.
Grossly visible folds or wrinkles present at the periphery of the tissue were removed using a sterile NF scalpel blade.
The tissue was processed using a sterile homogenizer, and serial 10-fold dilutions were plated via a spiral plater and enumerated as above.
Nematode infected root tissue was excised using a sterile scalpel at 3 days post-inoculation (dpi) and 9 dpi, respectively, under a magnifying glass and flash frozen immediately in liquid nitrogen.
Following the microscopic view, parts of unstained paraffin sections containing tumour tissues were scraped using a sterile surgical blade under the microscope and used for DNA extraction.
For genotyping, about 3 5 mm of tail tip (soft tissue only) was clipped using a sterile, sharp scalpel.
