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In six healthy adult horses, six full-thickness skin wounds were created on the dorsal aspect of both metacarpi using a sterile template.
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After cells reached 90% confluence, cross lines were generated using a sterile pipette tip.
The monolayer was mechanically wounded using a sterile 10 μl pipette tip.
The pericranium was removed using a sterile cotton swab.
Feces samples were taken during processing using a sterile catheter.
Using a sterile surgical blade no.
Under sterile conditions, tissue was washed 2× in 1× PBS and diced using a sterile blade.
Expressed breast milk (EBM) was obtained using a sterile expressing kit into sterile plastic pots.
The heat fixed slide material was emulsified using a sterile swab and sterile distilled water.
Use a sterile milk straw or clean, sterile dipper.
Use a sterile vial and fill about three-fourths full.
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