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The surface of MHA was completely inoculated using a sterile swab, which steeped in the prepared suspension of bacterium.
A bacteria culture (which has been adjusted to 0.5 McFarland standard), was used to lawn Muller Hinton agar plates evenly using a sterile swab.
The surface of the nutrient agar was completely inoculated using a sterile swab, which was steeped in the prepared bacterial suspension.
The surface of the Mueller-Hinton Agar was completely inoculated using a sterile swab, which was steeped in the prepared suspension of bacteria.
Pus was collected from abscesses by needle puncture or from necrotic material using a sterile swab, and cultured using standard culture methods.
Tissue samples were evaluated by qualitative aerobic and anaerobic culture in a CLIA-certified laboratory as follows: An unweighed portion of the sample was homogenized in a 15 ml conical tube using a sterile swab.
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Eye swabs were collected using a sterile cotton swab (Sterilin Ltd ,UK).
Using a sterile cotton swab the broth culture was uniformly swabbed on MHA.
Vaginal secretions were collected by using a sterile Dacron swab during the pelvic examination, and the swab was used to inoculate the InPouch TV (BioMed Diagnostics, San Jose, CA, USA) culture media, according to manufacturer's specifications.
After the incubation period, the fungi were swabbed on the agar plate using a sterile cotton swab.
The plastic jerry were cut open and then surface swabs for collecting microorganisms were done using a sterile cotton swab buds pre-wetted in peptone water at an area of 5 × 5 cm in three replicates.
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