Sentence examples for using a sterile pipette from inspiring English sources

After cells reached 90% confluence, cross lines were generated using a sterile pipette tip.

After 16-h serum starvation, 1-mm width 'scratch' wounds were created by scraping confluent cell monolayers using a sterile pipette.

The bottoms of the wells were sealed by adding a drop of molten agar into the wells using a sterile pipette.

By using a sterile pipette, 0.6 mL of the broth culture of each test organism was added to 60 mL of molten agar, which was cooled at 45 °C, mixed well and poured into a sterile Petri plate.

Colonies were collected and re-suspended in 0.85% NaCl to obtain a MacFarland turbidity of 2. A 100-μl aliquot of the turbid solution was thoroughly mixed with 7 ml API 20 C medium ampoule 2, and the resultant mixture was transferred to API 20 C AUX and API 5 CH cups using a sterile pipette.

The cell monolayer was wounded using a sterile pipette tip and washed twice with PBS to remove cell debris.

Use a sterile pipette to add a few drops of the microorganism solution to the agar.

We added 250 µL lysis buffer (0.1 M Tris-Hcl, pH 8.0, 0.1 M EDTA, 10 mM NaCl, 0.5% SDS) and used a sterile pipette tip to macerate the lesion, added 20 µL of Proteinase-K (final concentration, 1.0 mg/ml), and incubated at 65°C overnight (at least 6 h) before extraction with phenol/CHCl3/isoamyl alcohol (25∶24∶1).

Use a sterile pipette tip or inoculation loop to dip bacteria from the bacterial stab and streak bacteria onto LB Agar plates with proper antibiotics (see Reagents).

Three similar sized wounds were generated by scratching a gap using a sterile yellow pipette tip.

This was added to equal the volume of 2×concentrated BBS using a sterile Pasteur pipette.