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At the end of the study (d 42), one representative broiler chicken per pen, close to the average body weight, was selected for blood sampling using a sterile needle and heparinized vacuum tube.
Inoculation was performed using a sterile needle to puncture the middle of the agar plate.
The larvae were injected with sample solution between the sixth and seventh abdominal segments using a sterile needle.
The bone marrow cells were harvested from one end using a sterile needle.
Briefly, microdissection was performed under direct observation with an inverted microscope using a sterile needle.
Amniotic fluid was removed from embryos prior to dissection of limb buds using a sterile needle and syringe.
Similar(43)
Use a clean injection site and use a sterile needle for all injections.
To perform motility assay, single isolated colony was deep stabbed to a LB agar tube containing TTC using a sterile inoculating needle and incubated at 37 °C until growth was evident.
A 0.025% sterile collagenase solution (Boehringer, Mannheim, Germany) was injected through the apical opening into the dental pulp cavity by using a sterile 29G needle and incubated at 37°C for 30 minutes.
Inoculation was administered intra-cisternally through the teat canal using a sterile blunt needle.
Marked areas were manually macro-dissected under a microscope using a sterile injection needle (size 0.65 × 25 mm).
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