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The spores were dislodged using a sterile inoculation loop under strict aseptic conditions, and the number of spores in the suspension was counted using a Neubauer chamber.
Conidial suspensions were prepared by adding 10 ml of 0.1% Tween 80 (AppliChem) into the 4-week-old Petri dishes, and the conidia were taken from the agar surface, using a sterile inoculation loop to dislodge.
Using the micropipette, 100 μl of 0.5 McFarland solutions of bacterial strain cultures in 0.9 % sterile distilled water (SDW) was dispensed over the surface of an agar plate and spread using a sterile inoculation loop.
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Nematode infected root tissue was excised using a sterile scalpel at 3 days post-inoculation (dpi) and 9 dpi, respectively, under a magnifying glass and flash frozen immediately in liquid nitrogen.
Inoculation was performed using a sterile needle to puncture the middle of the agar plate.
Inoculation was administered intra-cisternally through the teat canal using a sterile blunt needle.
After inoculation 8.2 mm diameter wells were cut into the surface of the agar using a sterile cork borer.
The monolayer was mechanically wounded using a sterile 10 μl pipette tip.
The pericranium was removed using a sterile cotton swab.
Feces samples were taken during processing using a sterile catheter.
Using a sterile surgical blade no.
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