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Marked areas were manually macro-dissected under a microscope using a sterile injection needle (size 0.65 × 25 mm).
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Each intratesticular injection was performed using a sterile 27-guage 1/2 in.
Intramuscular injections were administered using a sterile 30-gauge, 0.5 inch needle and 0.2 ml (saline dilution) of onabotulinum toxin A (5 U) or placebo at each site, except for the trapezius where we administered 0.4 ml of onabotulinum toxin A (10 U).
The injections were given using a sterile ultrasound-guided technique into the region of maximal muscle injury, as determined by the initial MRI.
The injections were performed using a sterile ultrasound-guided technique into the region of maximal muscle injury, as determined by the initial MRI of the injury.
Hence, safe injection practice involves administration of rational injection by a qualified and well trained person using a sterile device (syringe, needle etc), adopting sterile technique, and discarding the used devices in a puncture-proof specially designed container for appropriate disposal.
The India ink was filtered using a sterile cellulose acetate membrane (0.2 μm pores, Renner GMBH, Darmstadt, Germany) just before injection.
The experimental solutions were given in ovo by injection into the albumen (at two-thirds of the egg's height from the blunt end) using a sterile 1-ml insulin syringe.
Using a sterile surgical blade no.
Under sterile conditions, tissue was washed 2× in 1× PBS and diced using a sterile blade.
Expressed breast milk (EBM) was obtained using a sterile expressing kit into sterile plastic pots.
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