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One hundred microliter samples were transferred onto the LB nutrient agar plates and sprayed evenly on top of the plates using a sterile glass rod.
Thereafter, the larvae belonging to the different groups were separately placed into glass test tubes (10 ml) including 3 ml nutrient broth (Difco, NJ, USA) with sterile forceps and completely homogenized, using a sterile glass tissue grinder.
The shed dried leaves were ground separately by commercial grinder (Hammer mill) into a fine powder and about 120 g of each powered materials macerated with methanol and occasional shaking in 900 mL methanol in a beaker at 25 ± 2 °C for 72 h and stirred every 18 h using a sterile glass rod.
The suspension was passed through a metal sieve, the larger pieces of tissue being disrupted further using a sterile glass pestle.
Corneal impression cytology specimens were collected using a sterile glass slide (Blue Star Micro Slides, Polar Industrial Corporation, Bombay, India).
Mycelium was then scrapped off the surface of the plates using a sterile glass slide, immediately frozen in liquid nitrogen, and lyophilized overnight.
Similar(43)
For lung homogenates, excised lungs were ground using a sterile glass-Teflon® in 500 µl PBS.
Positive samples were then scraped up using a sterile pipette tip, mixed with glass beads for 1 min to break open the host cells, centrifuged at 110×g for 5 min to remove any cell debris, and then the supernatant was added to an equal volume of storage buffer (4SP) and stored at −80°C.
Using a sterile pipette, 0.1 ml of the 10-2 dilution was introduced on the surface of sterile nutrient agar and a sterile glass spreader was used for even distribution of the inoculum.
The biofilm was thoroughly scraped using a sterile cell scraper (BD Falcon) and transferred to reagent tubes containing 4 mL sterile saline and about 30 (3 mm) sterile glass beads.
The biofilm was thoroughly scraped using a sterile cell scraper (BD Falcon, Bedford, MA, USA) and transferred to reagent tubes containing 4 mL sterile saline and about 30 (3 mm) sterile glass beads.
More suggestions(15)
using a sterile cotton
using a sterile borer
using a sterile scalpel
using a sterile probe
using a sterile toothpick
using a sterile water
using a clear glass
using a colored glass
using a sterile stir
using a sterile needle
using a magnifying glass
using a sterile stirrer
using a normal glass
using a sterile catheter
using a sterile template
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