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Samples were collected using a sterile container that was refrigerated (4°C), transported in an ice-pack to the medical laboratory, and processed within 1 hour of collection.
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A spot urine sample was collected between 9 00 am and 2 00 pm using a sterile polystyrene container (100 ml).
Mat pieces were collected using a sterile sampler and placed into sterile containers and stored at 4°C.
Moreover, after instructing how to collect urine specimen, about 20 ml of a clean catch morning mid-stream urine was collected from each patient [ 8] using a sterile screw-capped, wide-mouth container and labeled with the unique sample number, date and time of collection.
Grab sediment samples were collected from the top 5 cm of the riverbed at each sampling site, using a sterile polypropylene scoop and transferred to sterile 100 mL polypropylene containers with lids.
Cervical swabs were collected using a sterile flocked swabs (Copan, Italy) and transferred into one-tube DNA purification system (OTP Reagent, Bioron, Germany) container.
The remaining 20 mL of blood was collected in a sterile container containing EDTA.
Five millilitres of saliva were collected in a sterile container using aseptic techniques and stored at 4°C until used.
Ten millilitres of urine were collected in a sterile container using aseptic techniques and stored at -20°C until used.
The placental tissue samples will then be washed in a sterile container using ice cold, sterile phosphate-buffered saline.
Approximately 2 mL of unstimulated whole saliva was collected in a sterile container using the spit-out method.
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