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A novel environmentally sound continuous-flow hot water extraction and enzymatic hydrolysis method for determination of quercetin in onion raw materials was successfully constructed using a stepwise optimization approach.
Using a stepwise optimization, an overall whole-cell biocatalytic process was developed and a maximum titer of 260.0 mM Neu5Ac (80.4 g/L) with a conversion yield of 43.3% from GlcNAc was achieved.
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By using a stepwise manner, the whole moving process is shown as follows.
Using a stepwise regression model we determined the variables that best explained the jay density.
Models were defined using a stepwise ordinary least square (OLS) regression.
By using a stepwise method the most promising reactor type was chosen to meet the requirements.
Model selection was determined using a stepwise procedure.
Minimized systems were heated up by using a stepwise approach.
The plots were obtained using a stepwise (constant) model.
Two selected Trichoderma strains (Trichoderma sp.1 and Hypocrea neorufa.1) with high lipase producing potential were employed in a stepwise optimization.
We formulate the optimization problem, analyze its hardness, and solve it by designing VMPlanner as a stepwise optimization approach with three approximation algorithms.
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