Sentence examples for using a stepwise gradient from inspiring English sources
Each sample (20 μl per well) was measured between 25 and 90°C using a stepwise gradient of 0.5°C per 5 s.
Fraction C (11 g) was subjected to MPLC using a stepwise gradient of MeOH/H2O (0→100 %) to give fractions C1 C4.
The separation was performed using a stepwise gradient elution of 0 14 min, 95 87% B; 14 25 min, 87 87% B; and 25 45 min, 87 81% B; then keeping 95% B to balance for 10 min.
Fractions were eluted using a stepwise gradient of solvent B (66% acetonitrile in solvent A) (0%–55% B for 26 min, followed by 55%65%% B over 20 min, and 65%100%% B over 14 min).
Cy labelled adult fly sample mixtures were applied to the IPG strips (24 cm, pH 3 11, GE Healthcare) and isoelectric focusing was performed on an IPGphor II (GE Healthcare) at 20°C using a stepwise gradient.
However, Adebiyi et al. (2008) fractionated the rice bran hydrolysates to pure antioxidative peptides by RP-HPLC on a Kaseigel ODS resin, using a stepwise gradient of aqueous ethanol only.
Elution using a stepwise concentration gradient was able to produce fractions of reduced diameter population from the polydisperse HiPCO starting material, where a good correlation between the concentration of elution and local bond curvature for each nanotube species was observed.
For selective enrichment of marker proteins we used small sized anionic exchange spin columns resulting in 6 fractions after elution using a stepwise pH gradient from pH 9 3 and an organic fraction.
Samples were analyzed using a nanoAcquity UPLC system (Waters GmbH) connected online to a LTQ-Orbitrap Velos Pro instrument (Thermo Fisher Scientific GmbH) as described by Ori et al (1999) Briefly, peptides were separated on a BEH300 C18 (75 μm × 250 mm, 1.7 μm) nanoAcquity UPLC column (Waters GmbH) using a stepwise 145 min gradient between 3 and 85% (v/v) ACN in 0.1% (v/v) FA.
Peptides were eluted at a flow rate of 0.8 mL/min using a stepwise buffer B (0.1% NH4OH, 60% acetonitrile, pH 10.5) gradient.
By using a stepwise manner, the whole moving process is shown as follows.
