Sentence examples for using a step gradient from inspiring English sources

Elution of the alkaloids was effected using a step gradient of CH2Cl2-isopropanol (97 3), CH2Cl2-isopropanol (93:7), and then CH2Cl2-isopropanol (75:25).

Trypsin-like enzymes were then eluted from the column using a step gradient of NaCl with different concentrations ranging from 100 to 500 mM NaCl in the starting buffer.

An SFE trap system using C1 stationary phase connected to a C18 analytical HPLC column enabled the SFE LC MS MS analysis of three polyether ionophore antibiotics in water using a step gradient.

The proteins were eluted using a step gradient method including 40, 60, 100, 300 and 500 mM imidazole in buffer-A.

The chromatography was carried out using a step gradient from 0 25% solvent B in 33 min, from 25 40% solvent B in another 32 min and finally from 40 100% solvent B in 5 min. All experiments were performed on an Ultimate 3000 HPLC-system in µ-configuration (Dionex Softron GmbH, Germering, Germany) using a flow rate of 40 µL/min.

Proteins were then eluted from the heparin-agarose column using a step gradient of NaCl in buffer A. Protein fractions were pooled according to their molecular weight pattern of SDS-PAGE separation and concentrated using AMICON concentrator centrifugal devices with molecular weight cutoff of 10,000 kDa.

The residue was purified by chromatography using a stepped gradient of 0 15% MeOH in DCM.

The residue was purified by chromatography using a stepped gradient of 5 10% NEt3 in EtOAc, then 1 10 89 MeOH/NEto/EtOAc to give 43 mg of 6 as a yellow oil.

The residue was purified by chromatography using a stepped gradient of 0 8% EtOAc in heptane to yield 24 as a white solid.

The residue was purified by chromatography using a stepped gradient of 10 50% EtOAc in heptane to yield 26 as a white solid (88 mg, 51%).

The residue was purified by chromatography using a stepped gradient of 20 40% EtOAc in heptane to yield 41 as red brick solid.