Sentence examples for using a step elution from inspiring English sources

LacZ1 8Met-MTGase was eLacZ1 8Met-MTGasep elution wash 0.5 M sodium chloridelutedM sodiusingetate, pH 5.5.

Proteins were eluted using a step elution of imidazole in lysis buffer (50, 100, 150, 2 × 250 mM Imidazole).

The Ni-NTA column was washed twice with 10 ml of lysis buffer containing 30 mM imidazole, and the protein was eluted using a step elution of imidazole in lysis buffer (50, 100, 150, 2 × 250 mM imidazole in 50 mM HEPES [pH 7.5] at 25°C, 500 mM NaCl).

The target protein was eluted with the same buffer containing 1 M imidazole using a step elution protocol.

These strongly polar compounds can be removed from the HPLC column using either a step elution with the mobile phase of higher elution strength than n-hexane or by backflush.

Elution of the alkaloids was effected using a step gradient of CH2Cl2-isopropanol (97 3), CH2Cl2-isopropanol (93:7), and then CH2Cl2-isopropanol (75:25).

Elution of VraF was carried out using a step gradient of imidazole in Buffer A: 4 × 1.5 ml 100 mM imidazole, 4 × 1.5 ml 150 mM imidazole; 4 × 1.5 ml 200 mM imidazole and 4 × 1.5 ml 300 mM imidazole.

The mobile phase was 1 ml min−1, using a step-wise gradient elution program: %MeOH/0.005 M tetrabutylammonium chloride pH 7.4; 10/90–50/50 in 27 min. Over the range of 5 100 μg ml−1, the regression coefficient of the calibration curves (plasma and dialysate) were >0.998.

The increased purity observed with the QXL step supports the original rationale for switching from a step elution (original process) to a gradient elution (optimized process).

In addition, changing the Q Sepharose XL elution from a step elution to a gradient elution increased the protein purity without introducing a completely new variable so as to keep the changes minimal.

To also target extremely small <3 kDa peptides and simultaneously exclude >20 kDa proteins, we switched to reversed phase SPE cartridges for desalting and used a three-step elution to sequentially elute peptides by their charge for a higher purification and enrichment of basic peptides.