Sentence examples for using a step dilution from inspiring English sources
This DNA was reconstituted into a nucleosomal template with recombinant Xenopus octamers using a step dilution protocol and immobilized on streptavidin conjugated Dynabeads (Dynal) (11).
A relatively short piece of biotinylated DNA, 520 base pairs, is mixed in a 1 1 molar ratio (1 nucleosome per 175 basepairs of DNA) with purified recombinant Xenopus histone octamers (13) and reconstituted into a nucleosomal array using a step dilution protocol (11).
The N Latex assay was subjected to a prereaction step for 2 minutes prior to the assay, which allowed using a step with a higher dilution factor in the presence of a large amount of antigens, thereby reducing the false negatives caused by the antigen excess.
Therefore if a protein is sensitive to dilute acids, it is possible to deposit it using a PBS dilution step.
Working solutions used for the analysis in LC MS/MS or for the creation of urine samples fortified were obtained starting from stock solutions by dilution with water or methanol, using a dilution factor less than 10 in each step of dilution.
Creatinine determination in complex biofluids has been previously performed by GC/MS with isotope dilution, using a derivatization step to increase analyte volatility.
Information on primers and probe sets used are in additional file 1. PCR amplification efficiencies (E = 10-1/slope) were determined using a 5-step 5 fold dilution standard curve (10 ng to 16 pg) and PBMC total RNA.
For each set PCR amplification efficiency (E = 10-1/slope) was determined using a 5-step 5 fold dilution standard curve (25 ng to 4 pg) and pooled PBMC total RNA from several donors.
Clonal efficiency was assessed using a limiting dilution assay.
On a log dose scale, using a dilution factor of 1.47 corresponds to steps of log(1.47) = 0.38 each.
