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Cells isolated from induced sputum were co-cultured with ESAT-6 and CFP-10 antigens using a standardized enzyme-linked immunospot (ELISPOT) assay (T-SPOT®.TB) in 101 consecutively recruited TB suspects or non-TB controls.
Anticardiolipin antibodies in medium to high titer levels were identified using a standardized enzyme-linked immunosorbent assay (ELISA) IgG >15 IgG phospholipids units (GPL) or IgM >25 IgM phospholipids units (MPL).
To confirm the diagnosis of APS in the participant, positive serology was defined as at least one of the following tests to be positive in two occasions: Anticardiolipin antibodies in medium to high titer levels were identified using a standardized enzyme-linked immunosorbent assay (ELISA) IgG >15 IgG phospholipids units (GPL) or IgM >25 IgM phospholipids units (MPL).
Every enzyme is then assigned a unique entry identifier using a standardized system specific to mycoCLAP (7).
The data was collected using a standardized questionnaire.
Symptoms were obtained using a standardized in-person interview.
MAIN OUTCOME MEASURE: Patient satisfaction using a standardized PSQ.
Psychometricians assessed cognition using a standardized battery of neuropsychological tests.
Two independent observers graded RNFL photographs using a standardized protocol.
Corneal arcus was detected using a standardized slit-lamp examination.
Clinical and demographic data were assessed using a standardized protocol.
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