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The cell viability was three times determined by live and dead cell discrimination assay using a standardized cell viability kit (BD Biosciences, USA).
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Using a standardized persister cell assay that was designed to mimic slow-growing oxygen-limited in vivo conditions, we challenged cells from the stationary growth phase with antibiotics under static incubation (see Materials and Methods).
Ubiquitin-positive accumulations were quantified with ImageJ (NIH, Bethesda, MD) using a standardized threshold and normalized to cell count.
CT26.WT murine colon carcinoma cells were injected intraportally in Balb/c mice after median laparotomy using a standardized injection technique.
Clinical and demographic data were assessed using a standardized protocol.
The two reviewers extracted data independently using a standardized form.
Data were extracted by two reviewers using a standardized form.
The data was collected using a standardized questionnaire.
using a standardized form.
Wu) independently using a standardized extraction form.
Biopsies were taken using a standardized protocol.
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