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GAD 65 kDa autoantibody (GAD65 Ab) and insulinoma-associated protein 2 autoantibody (IA2) were measured using a standardized assay protocol and a common serum calibrator developed by the NIDDK-sponsored standardization group.
The number of doublings in 20 hours was used as a measure of fitness, and was determined using a standardized assay.
Samples were analyzed for GAD65 and insulinoma-associated protein-2 diabetes autoantibodies (DAs) using a standardized assay protocol and a common serum calibrator developed by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK -sponsored staNIDDK -sponsoredup.
Autoantibody testing was performed using a standardized assay protocol and a common serum calibrator developed by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK -sponsored staNIDDK -sponsoredup.
Five alkaloids (canadine, coclaurine, stylopine, reticuline, pavine) were screened in triplicate as potential substrates for G. flavum NMTs using a standardized assay under linear product formation conditions (30 μg total protein, 100 μM alkaloid, 200 μM S-adenosyl methionine, 100 mM sodium phosphate, pH 7).
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The multiplex system we developed in this study is ideally suited to the simultaneous analysis of multiple biomarkers because it uses a standardized assay platform and is highly automated, allowing high-throughput reproducibility across clinical laboratories.
CSF samples were processed for the detection of M.tb using a standardized PCR assay (Roche AMPLICOR) as per manufacturer instructions.
Our study, using a standardized ELISPOT assay incorporating RD-1 specific antigens (ESAT-6 and CFP-10), confirms that rapid immunodiagnosis of M.tb infection is indeed possible using mononuclear cells from induced sputum, but demonstrates that due to technical factors its clinical utility is limited.
Testing was done by using a standardized microneutralization assay.
Protein concentration was measured using a standardized Bradford assay.
Quantification of mHLA-DR on circulating monocytes was performed using a standardized flow cytometric assay as previously described [24.] The median fluorescence intensity of the entire monocyte population was then transformed to number of antibodies bound per cell (ABC) using calibrated PE beads (BD QuantiBRITE™ PE Beads, Becton Dickinson San Jose, CA, USA).
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