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The SUVmax of each nodule was recorded using a standard volume of interest (VOI) tool.
The first extraction was performed using a standard volume of 2.0 mL Oragene·DNA/saliva mixed samples and the second one using the remaining volume of mixer in the collection vial.
In order to mimic slow-growing hypoxic in vivo conditions, the assay was carried out using stationary-phase cultures, which were distributed into 24-well plates (nonpyrogenic polystyrene; Corning) using a standard volume of 1 ml per sample containing approximately 10 CFU each.
DNA was isolated from each saliva sample using a standard volume, containing the recommended protocol minimum of 3.5 x 10 cells, using the GenomicPrep DNA isolation kit (Amersham Biosciences: Buckinghamshire, UK; now GE Healthcare), following the procedure recommended by the manufacturer as previously described [ 26- 28].
Using a standard volume for each gel well, samples were loaded from input, eluate, flowthrough, and wash fractions were loaded onto a 10% Bis/Trisacrylamide gel (BioRad), separated, transferred to nitrocellulose membrane, and incubated with anti-Thap1 or anti-V5 antibodies, as indicated.
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Since we used a standard-volume for all participants this adds imprecision to the mapping of the hubs.
In this paper, attention is limited to two-dimensional inviscid flows using a standard finite volume discretization, although the procedure may be readily applied to other types of multidimensional problems and discretizations.
Both treatments were administered intravenously (IV) once daily for 5 days (QDx5) using a standard dosing volume of 10 ml/kg body weight.
All treatments were administered intraperitoneally (IP) at one dose per day every fourth day for a total of 3 doses (Q4Dx3) using a standard dosing volume of 10 ml/kg body weight.
The air sampler was calibrated with a 2100 magnehelic pressure gauge using a standard high-volume sampler calibration procedure (Dwyer Instruments, Michigan City, IN).
Each volume was normalized using nonlinear basis functions and then resampled into 3 × 3 x 3 mm voxels, using a standard EPI template volume based on the Montreal Neurological Institute (MNI) reference brain (Cocosco et al. 1997) in the space of Talairach and Tournoux (1988; Ashburner and Friston 1999).
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