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First, we performed single-species tests to assess the toxicity of enrofloxacin under different temperature conditions (20 °C and 30 °C) and to assess the sensitivity of different species strains using a standard temperature (20 °C).
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To show that these hybridization results were similar to a solution phase hybridization, melting curve measurements were taken in solution using a standard temperature-controlled spectrophotometer.
Interventions: Intradiskal electrothermal therapy (IDET) by using a standard high-temperature heating protocol with the temperature of the probe gradually increased from 65°C to 90°C over 16.5 minutes.
All the analyses were conducted on samples collected using a standard sampling method, except for temperature measurements which were performed in situ.
pH values are measured in triplicate and at room temperature, using a standard Corning electrode.
In brief, 100 μg of protein was incubated in a 96-well plate with 50 μM luciferin and 1.25 μg/mL luciferase in a Tris-based 1X reaction buffer containing DTT. ATP was measured after 5 min using a luminometer (560 nm at room temperature) using a standard curve of known ATP concentrations.
The thermopower coefficient is measured as a function of temperature from room temperature down to liquid nitrogen temperature, 77 K, using a standard differential technique.
We have investigated the kinetics, rate oscillations and electrochemical promotion of CO oxidation on Pt deposited on YSZ using a standard oxygen reference electrode at temperatures 300 400°CC.
Room temperature was measured using a standard alcohol thermometer.
Water and air temperature was measured using a standard Celsius thermometer with the accuracy of ±0.5 °C.
The experiment was carried out in dark room at constant temperature of 25 °C using a standard hot surface as thermal source.
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