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We next consider the diversity of the designed sequence ensembles, using a standard sequence entropy [37], [78].
Since mapping of such different lengths of sequence containing the unmappable adapter sequence was not possible using a standard sequence mapping/assembly program, we utilized the property of mpsmap that maps different length sequences to the best chromosomal location, while allowing up to a specified number of mismatches without a gap.
Bright-blood images were acquired using a standard sequence employing a T2-preparation module followed by steady-state free-precession readout (T2-prep time, 80 ms; TR, 2 3 R-R intervals; flip angle, 60°; slice thickness, 7 mm; in-plane resolution, 1.1×1.0; bandwidth, 975 Hz/pixel).
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For diffusion-weighted imaging, we used a standard sequence (23 slices, matrix = 128 × 128 pixel, voxel size = 1.8 × 1.8 × 5 mm, repetition time = 3.1 s, echo time = 79 ms, flip angle = 90°, six diffusion-encoding gradient directions with a b-factor of 1000 s/mm).
Thus far, large resequencing projects have used a standard sequencing procedure in which gene fragments are amplified by PCR, purified and subjected individually to Sanger sequencing on both strands [ 1, 2].
Parts of the regulatory region from 2449 bp upstream to 374 bp downstream of the TNFSF4 transcription start site (2823 bp in total) and parts of intron 1 were PCR amplified and sequenced using a standard cycle-sequencing system (PTC-225, MJ Research, Waltham, MA, USA).
Moreover, the internal motion of the myocardium may not be measured correctly if its MR signals appear uniform using a standard GRE sequence.
The penetrating brain injury patients were scanned using a standard CT sequence since they had retained metal in their brain.
Data were acquired using a standard PRESS sequence with an echo time of 30 ms, a relaxation delay of 2,000 ms and 176 transients.
DNA barcoding can quickly and reliably identify species using a standard genetic sequence that can be amplified by universal primers from a small fragment of the genome [ 22– 25].
Bilateral single-voxel spectra were acquired using a standard PRESS sequence to provide a metabolite profile from three brain regions of interest (ROIs): basal ganglia and frontal and temporal lobes.
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