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Genomic DNA extraction was performed from the plasma-free blood pellets using a standard proteinase K and salt precipitation method.
DNA was isolated from tails using a standard proteinase procedure.
Briefly, DNA was isolated from G. flavescens using a standard proteinase K, phenol-chloroform procedure [ 51].
DNA was extracted from each subset using a standard proteinase K digestion and phenol/chloroform extraction.
Total DNA was isolated from each individual mite using a standard proteinase K-phenol chloroform method [ 33].
Genomic DNA from tissue homogenate was extracted using a standard proteinase K and phenol-chloroform method [ 16].
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DNA isolation was then carried out using a standard phenol chloroform plus proteinase K protocol.
Total genomic DNA was isolated using a standard protocol with proteinase digestion, phenol chloroform extraction and ethanol precipitation.
A few of the non-tumor reference cases were extracted using a standard method with proteinase K digestion, phenol chloroform extraction, and ethanol precipitation.
Genomic DNA was extracted from frozen whole blood samples using a standard cell lysis, proteinase K digestion, phenol/chloroform, ethanol precipitation method.
DNA extraction of the paraffin embedded tumours was done using a standard protocol with proteinase K digestion and phenol/chloroform extraction [ 11], which yielded between 3 and 15 μg DNA from one section.
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