We carried out immunoblotting using a standard protocol and primary antibodies against PICT1, TP53 (Dako, A/S, Glostrup, Denmark), CDKN1A, and BAX (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
Since then it has been used as a standard primary outcome measure in studies investigating the efficacy of pharmacological treatments.
We interviewed all consenting residents using a standard questionnaire in Karenni, their primary language, and collected a fecal sample and blood sample from them.
Briefly, cells were grown on coverslips, fixed in methanol at -20°C for five minutes and stained using a standard immunocytochemistry protocol with a primary monoclonal antibody that recognizes γ-tubulin (1 μg/mL; clone GTU-88, Sigma) and a secondary fluorescein isothiocyanate (FITC) conjugated sheep F AB' 2 fragment to mouse IgG (whole molecule) (ICN Pharmaceuticals, Inc., Costa Mesa, CA, USA).
The mixed form of Richard's equation is solved using a standard finite element method (FEM) with primary variable switching.
Although we standardised primary data using a standard portion size of 106 g, conclusions should be interpreted with caution.
The eluates were analysed by western blotting, using a standard concentration of 1 : 1000 of the primary antibody.
In our primary experiments using a standard MTT (3- 4,5 -dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) cytotoxicity assay, we observe that a 48-h preincubation of Raji, Ramos or Daudi cells (Burkitt's lymphomas), as well as DoHH2 cells (follicular lymphoma), with sorafenib significantly potentiates the ability of rituximab to induce complement-dependent cytotoxicity (CDC; Figure 1a).
Progression status is a standard primary endpoint used in clinical trials to define efficacy, and as such has been the obvious choice for defining health states in cost-effectiveness modelling.
Controls for IHC: For each primary antibody used, a standard control was performed in which the primary antibody was omitted from the protocol (to control for non-specific signal resulting from subsequent antibody and signal-detection reagents).
