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The absorbance was calculated at 570 nm using a standard plate reader.
Viability assays of P. aeruginosa and S. aureus cells were performed using a standard plate count assay [29].
This is achieved without muscle violation and has the added benefit of using a standard plate and screw fixation device, which is available in most hospitals worldwide.
b The correlation between bactericidal efficiencies (evaluated over a period of 3 h by using a standard plate count method) and surface characteristics (topological parameters and water contact angles).
Fluorescence, which correlates with cytoplasmic ROS accumulation, was detected using a standard plate reader.
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Raw data from these screens were normalized using a standard plate-based Z-score analysis.
The MTT assay was performed as described (Hansen et al. 1989) in 96-well format, and the O.D. at 570nm was measured using a standard plate-reader.
The A549 cell lysate was then diluted and plated on sheep blood agar (SBA) (Remel), using a standard spread plate technique.
The absorbance (450 nm) of each sample was analyzed using a standard ELISA plate reader.
EL-CCD detector measured limits of detection (LODs) were similar to those measured using a standard fluorescent plate reader with valves between 0.625 and 1.25 nM (31 62 ng/ml) for LcA and 0.313 nM (45 ng/ml) for the full toxin, BoTN-A.
This was at least an order of magnitude better than that observed using a standard microtiter plate ELISA with the same Ab reagents.
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