Sentence examples for using a standard pcr from inspiring English sources

PCR reactions were performed using a standard PCR pre-mix [ 11].

qRT-PCR was performed on 1 μl 20x diluted cDNA, using a standard PCR reaction mix for Phusion DNA polymerase (Finnzymes), with the addition of 1.25 μL 500x diluted SYBR Green (BioRad®) in DMSO.

Candidate markers in this minimum set were then tested for their amplifiability using a standard PCR protocol to establish their practical usefulness as new, objectively inferred phylogenetic markers.

This involved amplification of the targeted loci using a standard PCR protocol followed by digestion with the restriction enzymes PstI (NEB #R0140S) for rs12845494, and BsmAI (NEB #R0529L) for rs2506142.

Microsatellites were amplified using a standard PCR protocol [30].

Since exon 1 and majority of exon 11 of PIGR gene do not contain any coding sequence, we only amplified exon 2 though 10 and the first fragment of exon 11, using a standard PCR protocol.

The deletion strains generated for this study were designed using a standard PCR-mediated gene insertion technique (Longtine et al. 1998) and confirmed by PCR analysis using two sets of primer pairs (sequences available upon request).

All constructs and point mutations were generated using a standard PCR-based cloning strategy and verified through DNA sequencing.

The previously described cDNA clone of human HSL [8] was modified using a standard PCR-based mutagenesis protocol, cloned into the pVL1393 shuttle vector from PharMingen (BD Biosciences) and sequenced.

All constructs and point mutations were generated using a standard PCR-based cloning strategy.

These assays use a standard PCR format where all the assays are performed with the same PCR cycling conditions.